Chinese Name: 5-Bromo-6-chloro-3-indolyl N-acetyl-β-D-glucosaminide

English Name: 5-Bromo-6-chloro-3-indolyl N-acetyl-β-D-glucosaminide

Abbreviation: Magenta-GlcNAc

CAS No.: 5609-91-6

Molecular Formula: C16H18BrClN2O6

Molecular Weight: 449.68

Specification: 1 g / 25 g / 100 g (Custom packaging supported)

Form: Lyophilized powder

Catalog No.: G01020007

Stock: In stock

Color Developed: Magenta

β-N-acetylglucosaminidase (NAGase) specifically hydrolyzes its glycosidic bond, releasing 5-bromo-6-chloro-3-indoxyl. This product is then oxidized by an oxidant (such as ferricyanide) present in the system and undergoes coupling reactions, generating an insoluble magenta-colored precipitate in situ, thereby visually and stably indicating the active sites of NAGase.

✅ High Specificity
Specifically recognizes NAGase (β-N-acetylglucosaminidase) with no cross-reactivity to α-configured enzymes, ensuring accurate and reliable detection.

✅ Clear Chromogenic Signal
The enzymatic product is an insoluble magenta precipitate with high contrast, suitable for visual observation and image analysis.

✅ In Situ Deposition

The precipitate does not diffuse, making it suitable for scenarios requiring spatial localization such as tissue section staining and colony screening.

✅ Lyophilized Stability
Excellent stability in powder form, effectively avoiding hydrolysis in solution and facilitating long-term storage.

Microorganism Identification: Specifically differentiate bacteria or fungi with NAGase activity.

Clinical Diagnosis: Widely used in the preparation of rapid chromogenic media for Candida albicans.

Basic Research: Used for in situ chromogenic analysis in enzymatic studies or histochemical staining.

Food Safety: Assist in the detection of specific pathogenic bacterial contamination.

Q1: What is Magenta-GlcNAc? Which enzyme does it detect?
A: This product is an indoxyl chromogenic substrate specifically designed for detecting β-N-acetylglucosaminidase (NAGase). This enzyme is a key marker for lysosomal function and various microorganisms (such as Candida albicans).

Q2: What is the detection (chromogenic) principle of Magenta-GlcNAc?
A: NAGase specifically hydrolyzes the glycosidic bond of the substrate, releasing 5-bromo-6-chloro-3-indoxyl. In the presence of an oxidant (such as ferricyanide), this product is rapidly oxidized and coupled in situ, forming an insoluble magenta precipitate, thereby enabling visual localization of enzyme activity.

Q3: What is the core difference compared to common colorimetric substrates like pNP-GlcNAc?
A: The core difference lies in the form of the chromogenic product:
- pNP-GlcNAc → Soluble yellow product, suitable for solution quantification
- Magenta-GlcNAc → Insoluble magenta precipitate, formed in situ
Therefore, Magenta-GlcNAc is more suitable for application scenarios requiring spatial resolution, such as colony screening, tissue localization, and multi-target chromogenic detection.

Q4: What are the main application areas?
A: Magenta-GlcNAc is primarily used for detecting β-N-acetylglucosaminidase (NAGase) activity. Its core applications include: microorganism identification (specific chromogenic identification of enzyme-producing species, such as Candida albicans); clinical diagnosis (preparation of rapid chromogenic media for Candida); basic research (enzymatic studies or in situ chromogenic analysis);

Q5: Is this substrate suitable for multiplex chromogenic or combined detection?
A: Yes. Magenta-GlcNAc is often used as one color node in a multiplex chromogenic solution. It can be combined with other differently substituted indoxyl glycoside substrates to achieve multi-target, multi-color parallel detection through structural differences.

Q6: Is the chromogenic background prone to be high? Will it affect interpretation?
A: This substrate belongs to a precipitate-based chromogenic system with a low natural hydrolysis rate and a clean background.
Coloration is mainly confined to the enzyme action sites, with low diffusivity and clear boundaries, which helps reduce false positives and improve result interpretation consistency.

Q7: What packaging specifications are available? Is customization supported?
A: Three standard specifications are available: 1g (trial), 25g (regular), 100g (large scale). Custom packaging is supported to meet needs at different R&D or production stages.

Q8: Is this product suitable for developing detection kits?
A: Highly suitable. This product features high batch-to-batch stability, low background, and clear coloration, making it an ideal core raw material for developing NAGase activity detection kits (especially histochemical or microbial identification kits requiring in situ coloration).