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In Stock Series | Simplifying β-D-Galactosidase Detection: A One-Stop Solution from Chromogenic to Fluorescent Substrates
2026/1/14 16:18:39 Browse volume(24)β‑D‑Galactosidase (β‑Gal / LacZ)
β‑D‑Galactosidase (β‑D‑Galactosidase, abbreviated as β‑Gal / LacZ) specifically hydrolyzes β‑D‑galactosidic bonds, making it one of the most classic marker enzymes in molecular biology and microbiology. Based on specialized substrates for β‑Gal , researchers can convert enzyme activity into visible color or fluorescent signals: enabling colony coloration and rapid interpretation on plates/medium, or performing more sensitive signal reading and quantitative analysis in microplate systems.
GeneSeqTools Bioscience & Technology provides a series of β‑Gal substrates covering colorimetric and high-sensitivity detection (readily available, multiple specifications, supporting customized packaging), meeting different readout needs for R&D and large-scale applications.
β-D-Galactosidase is a glycoside hydrolase that specifically hydrolyzes β-D-galactosidic bonds and is widely present in various bacteria, with E. coli and Enterobacteriaceae being the most typical examples.
In the field of microbiological detection, β-D-galactosidase activity is often used as a metabolic phenotype indicator to differentiate microbial populations or assist in species identification. β‑Gal substrate-based detection offers three typical advantages:
1. Specificity: Substrate design targets β‑D‑galactosidic bonds, offering more controllable background.
2. Visualizability: Colorimetric substrates can directly present colony color.
3. Quantifiability: Fluorescent/red signal substrates can be used for low-abundance detection, microplate assays, and high-throughput screening.
How β-D-galactosidase substrates generate signals hinges on the introduction of a signal group in their molecular structure that responds to enzymatic cleavage. β-D-galactosidase substrates typically consist of signal group + β-D-galactosidic bond:
· Before the reaction:
The substrate molecule is intact, showing no color or fluorescence signal.
· Under the action of β-D-galactosidase:
The β-D-galactosidic bond is specifically cleaved, releasing indole-based or fluorescent signal molecules.
· After the reaction:
For colorimetric substrates, further oxidation or coupling forms a stable colored precipitate;
For fluorescent substrates, high signal-to-noise ratio fluorescence signals are released.
This reaction system has strong specificity and low background interference, making it suitable for the construction of chromogenic media and the development of enzymatic activity detection methodologies.
1. X-Gal
Chinese Name: 5-Bromo-4-chloro-3-indolyl β-D-galactopyranoside
English Name: 5-bromo-4-chloro-3-indolyl β-D-galactopyranoside
Abbreviation: X-Gal
CAS No.: 7240-90-6
Molecular Formula: C14H15BrClNO6
Molecular Weight: 408.63
Specifications: 1 g / 25 g / 100 g (custom packaging supported)
Form: Lyophilized powder
Catalog No.: G01010003
Stock: In stock
Color Development: Blue
Application: For the detection of microorganisms producing β-D-galactosidase, enabling intuitive color interpretation of target colonies in chromogenic media or solid culture systems.
2. Magenta-Gal
Chinese Name: 5-Bromo-6-chloro-3-indolyl β-D-galactopyranoside
English Name: 5-Bromo-6-chloro-3-indolyl β-D-galactopyranoside
Abbreviation: Magenta-Gal
CAS No.: 93863-88-8
Molecular Formula: C14H15BrClNO6
Molecular Weight: 408.63
Specifications: 1 g / 25 g / 100 g (custom packaging supported)
Form: Lyophilized powder
Catalog No.: G01020003
Stock: In stock
Color Development: Magenta
Application: Suitable for the chromogenic identification of β-D-galactosidase-positive bacteria, and can serve as a differentiated signal substrate in multi-color chromogenic media.
3. Y-Gal
Chinese Name: 3-Indolyl β-D-galactopyranoside
English Name: 3-Indolyl β-D-galactopyranoside
Abbreviation: Y-Gal
CAS No.: 126787-65-3
Molecular Formula: C14H17NO6
Molecular Weight: 295.29
Specifications: 1 g / 25 g / 100 g (custom packaging supported)
Form: Lyophilized powder
Catalog No.: G01040006
Stock: In stock
Color Development: Dark blue
Application: For β-D-galactosidase activity detection and chromogenic media development, suitable for detection systems requiring stable chromogenic reactions.
4. Salmon-Gal
Chinese Name: 6-Chloro-3-indolyl β-D-galactopyranoside
English Name: 6-Chloro-3-indolyl β-D-galactopyranoside
Abbreviation: Salmon-Gal
CAS No.: 138182-21-5
Molecular Formula: C14H16ClNO6
Molecular Weight: 329.73
Specifications: 1 g / 25 g / 100 g (custom packaging supported)
Form: Lyophilized powder
Catalog No.: G01030003
Stock: In stock
Color Development: Salmon pink
Application: For β-D-galactosidase chromogenic detection, providing easily distinguishable warm-color signals in multi-target or multi-substrate systems.
5. Resorufin-Gal
Chinese Name: Resorufin β-D-galactopyranoside
English Name: Resorufin β-D-galactopyranoside
Abbreviation: Resorufin-Gal
CAS No.: 95079-19-9
Molecular Formula: C18H17NO8
Molecular Weight: 375.33
Specifications: 1 g / 25 g / 100 g (custom packaging supported)
Form: Lyophilized powder
Catalog No.: G01050001
Stock: In stock
Color Development: Red fluorescence
Application: For fluorescent detection and quantitative analysis of β-D-galactosidase, suitable for methodology validation and high-sensitivity detection systems.
6. 4MU-Gal
Chinese Name: 4‑Methylumbelliferyl β‑D‑galactopyranoside
English Name: 4-Methylumbelliferyl β-D-galactopyranoside
Abbreviation: 4MU-Gal
CAS No.: 6160-78-7
Molecular Formula: C16H18O8
Molecular Weight: 338.31
Specifications: 1 g / 25 g / 100 g (custom packaging supported)
Form: Lyophilized powder
Catalog No.: G01070001
Stock: Backorder
Color Development: Blue fluorescence
Application: For quantitative detection of β-D-galactosidase activity, with fluorescence intensity showing good correlation with enzyme activity, suitable for instrument-based detection platforms.
✅ In-Stock Supply: Multiple β-D-galactosidase substrates are readily available, supporting various specifications and custom packaging.
✅ Clear Targeting: Specifically responsive to β-D-galactosidase activity with low background interference, suitable for microbial enzymatic phenotype detection systems.
✅ Intuitive Signals: Colorimetric reactions enable rapid visual interpretation, while fluorescent signals allow instrumental quantification, balancing efficiency and sensitivity.
✅ Mature System: β-Gal substrates have been long-term used in microbial chromogenic media and detection methods, featuring stable and reliable technical routes.
✅ Flexible Selection: Various chromogenic or fluorescent substrates can be flexibly chosen based on detection needs, adapting to multiple detection platforms and protocols.
Extended Services
Enzymatic cleavage and color development, instantly clear; Fluorescence quantification, revealing subtle details — A comprehensive solution for β‑galactosidase detection.
For more chromogenic substrates or customized packaging services, please call +86 13302967066!
GeneSeqTools Bioscience & Technology focuses on providing reliable domestic alternative solutions for gene sequencing, molecular diagnostics, and microbial detection.
For related products such as blood cell dyes and buffer salts, you can view the complete product line and technical specifications on the 【MedSun Biotechnology Website】.
Founded in 2015, it is a high-tech enterprise focusing on microbial testing、 gene sequencing and in vitro molecular diagnostics in the R&D, production, and sales of biochemical reagent raw materials. Committed to providing high-quality biochemical reagent raw materials and professional custom synthesis services, the product portfolio covers more than a dozen categories and nearly a thousand innovative products. Upholding the core philosophy of “customer first, integrity and professionalism”, we serve with dedication to meet customers’ diverse needs in research and testing.
Official WeChat account: Shenzhen GeneSeqTools Bioscience & Technology Co. Ltd.
GeneSeqTools official website: http://www.geneseqtools.com
Hotline: +86 0755-23251735
QQ: 2370561481
Email: sales@geneseqtools.com
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