NSP-DMAE-HEG-Glu-NHS belongs to the category of direct chemiluminescent labels. Its luminescence mechanism is simple, fast, and efficient:

1. Trigger Reaction: The acridinium ester molecule is instantly excited in an alkaline hydrogen peroxide (H₂O₂) solution.

2. Flash Release: The excited-state molecule rapidly decays, releasing intense blue light with a wavelength between 430~480 nm. The entire process is completed within 1~5 seconds, forming a typical "flash-type" luminescence.

3. Pure Signal: Before luminescence, the luminescent moiety (acridone) has already dissociated from the labeled antibody or antigen, so the signal is not interfered by the structure of the biomacromolecule, resulting in extremely low background noise.

4. Advantage of Small Molecule: Its small molecular weight means labeling has minimal impact on the binding activity and spatial conformation of antigens and antibodies, making it particularly suitable for competitive immunoassays and ensuring detection accuracy.

This principle endows it with innate advantages of high quantum yield, high signal-to-noise ratio, and rapid detection, making it an ideal choice for achieving high-sensitivity, high-precision immunoassays.

Chinese Name: N-Sulfopropyldimethylacrylidium Ester-Hexaethylene Glycol-Glutarate NHS Active Ester

English Name: N-Sulfopropyl-Dimethyl Acridinium-Ester Hexa(ethylene) glycol-Glutarate N-hydroxysuccinimide

Abbreviation: NSP-DMAE-HEG-Glu-NHS

CAS Number: 1253933-74-2

Molecular Formula: C47H58N4O16S

Molecular Weight: 967.05

Specifications: 1 mg / 5 mg / 10 mg (Custom packaging supported)

Form: Lyophilized Powder

Cat. No.: G05010005

Stock Status: In Stock

Color: Blue

✅ High Sensitivity Detection
Quantum yield is increased by approximately 50%, resulting in stronger signals, significantly enhancing the detection sensitivity for low-concentration analytes.

✅ Second-Flash Anti-Interference
1~5 second rapid flash, peak wavelength 430~480 nm matches the high-sensitivity region of PMT, effectively avoiding fluorescence interference from biological matrices.

✅ Stable and Long-lasting
Classic dimethyl steric hindrance design effectively prevents ester bond hydrolysis, endowing the reagent with excellent anti-hydrolysis capability and storage shelf life.

✅ Convenient Conjugation
Features a built-in NHS active group, allowing direct conjugation with protein amino groups without the need for additional activation steps. Small molecule labeling does not damage activity.