Oxidative damage in natural DNA occurs randomly, making precise localization studies difficult. However, chemically synthesized 8-oxo-dG phosphoramidite monomers enable researchers to introduce this modification at specific sites, achieving:

The 8-oxo-dG phosphoramidite monomer enables precise introduction of oxidative damage into DNA strands through solid-phase synthesis technology. It employs a triple protecting group design: 5'-DMT protection ensures synthesis direction control, isobutyryl protection maintains base stability, and β-cyanoethyl phosphoramidite ensures efficient coupling.

During DNA synthesis, the monomer specifically connects to the growing chain via the phosphoramidite group. After deprotection steps, the 8-oxo-dG modification is precisely embedded into the predetermined sequence. This design completely retains the biological characteristics of 8-oxo-dG, providing a molecular-level precise control solution for oxidative damage research.

Chinese Name: 2ʹ-Deoxy-5ʹ-O-DMT-isobutyryl-8-oxoguanosine 3ʹ-CE phosphoramidite

English Name: 2ʹ-Deoxy-5ʹ-O-DMT-isobutyryl-8-oxoguanosine 3ʹ-CE phosphoramidite

Abbreviation: 8-oxo-dG-CE Phosphoramidite

CAS Number: 143060-53-1

Molecular Formula: C44H54N7O9P

Molecular Weight: 855.93

Specification: 100 mg / 250 mg /1 g (Custom packaging supported)

Form: Off-white powder

Item Number: G09010022

8-oxo-dG-CE Phosphoramidite is a chemically modified nucleoside monomer that can be directly incorporated into oligonucleotide chains, and is a core research raw material for DNA oxidative damage simulation, mutation mechanism research, and repair enzyme recognition analysis.

1. DNA Oxidative Damage and Mutation Research: By synthesizing oligonucleotides containing 8-oxo-dG at specific positions, study their replication fidelity and mutation characteristics, revealing the genotoxic mechanism of oxidative damage.

2. DNA Repair Enzyme Kinetics Analysis: As a natural substrate for repair enzymes such as OGG1 and MUTYH, used for enzyme activity determination, inhibitor screening, and repair mechanism research.

3. Biomarker Detection Probe Development: Construct 8-oxo-dG specific detection probes for oxidative stress level assessment and environmental toxicology research.

✅ High Stability: Optimized protecting group (DMT/isobutyryl/CE) design ensures synthesis efficiency and structural integrity.

✅ Strong Synthesis Compatibility: Compatible with mainstream DNA synthesis platforms, easy to operate.

✅ Strict Quality Control: Strict batch testing and HPLC purity control ensure experimental consistency.

✅ Customized Services: Provide custom synthesis of modified oligonucleotides and technical support services.