In our previous content, we delved into how to use 8-oxo-dG-CE Phosphoramidite (143060-53-1) to precisely simulate oxidative damage at specific sites on DNA strands, helping researchers analyze the intrinsic mechanisms of diseases such as aging and cancer.

The charm of life science lies not only in 'understanding' but also in 'creation'. In this issue, we shift our focus from 'inward exploration' to 'outward construction'—how to actively endow oligonucleotides, the blueprint of life, with new functions, making them powerful molecular machines that can luminesce, target, and treat.

Natural oligonucleotide structures are stable and precisely recognizable, but to achieve broader research or therapeutic functions, it is necessary to introduce chemically controllable 'reaction sites' on them:

The key to achieving all this is to find an efficient, stable, and site-specific connection strategy.

Among numerous conjugation strategies, copper-free click chemistry (SPAAC) has become a mainstream solution due to its high selectivity, mild conditions, and biocompatibility.

The core reaction is the cycloaddition between the DBCO (Dibenzocyclooctyne) group and the azide (–N?) group, forming a stable triazole ring structure.

The entire reaction requires no metal catalyst, can proceed in aqueous phase and at room temperature, and does not disrupt biological activity or introduce toxic ions.

This makes DBCO an ideal interface for 'connecting biomolecules with functional molecules'.

The design logic of DBCO-dT-CE Phosphoramidite is precisely to 'pre-embed' this reaction site into the DNA strand.

During solid-phase DNA synthesis, researchers can insert DBCO-dT-CE Phosphoramidite into the target position just like using ordinary bases.

After synthesis is completed, this site carries a 'DBCO handle':

1. This handle can undergo click reactions with azide-modified molecules (such as dyes, biotin, PEG, or drugs);

2. The reaction conditions are mild (room temperature/aqueous solution) and require no copper ion catalysis;

3. Ultimately, a covalent and stable linkage is formed;

By presetting the DBCO site during DNA synthesis, precise and controllable molecular conjugation can be achieved.

DBCO-dT-CE Phosphoramidite acts like a 'universal interface' in oligonucleotides, making subsequent functional design more efficient and free.

Chinese Name: DBCO-dT-CE Phosphoramidite

English Name: 5'-Dimethoxytrityl-5-[(6-oxo-6-(dibenzo[b,f]azacyclooct-4-yn-1-yl)-capramido-N-hex-6-yl)-3-acrylimido]-2'-deoxyUridine,3'-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite

Abbreviation: DBCO-dT-CE Phosphoramidite

CAS Number: NA

Molecular Formula: C69H80N7O11P

Molecular Weight: 1214.41

Specification: 100 mg / 500 mg / 1 g (custom packaging supported)

Form: Off-white powder

Product Number: G09010021

DBCO-dT-CE Phosphoramidite is a key raw material for the functional construction of oligonucleotides. It can efficiently conjugate with various functional molecules through copper-free click chemistry and is widely used in probe, drug, and nanotechnology research.

1. Nucleic Acid Drug Development: Construct targeted ASO, siRNA, or Aptamer-drug conjugates (ApDC);

2. Molecular Diagnostic Probes: Construct FISH probes, real-time fluorescent probes, NGS library adapters;

3. Nanobiotechnology: DNA origami, molecular sensors, nanocarriers;

By presetting DBCO sites during nucleic acid synthesis, researchers can efficiently construct structurally precise and functionally diverse nucleic acid systems, significantly improving experimental efficiency and structural controllability.