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In Stock Series | NGS Nucleotide Building Block Iodo-dA: The “Molecular Foundation” Redefining Sequencing Stability

2025/11/20 14:21:07 Browse volume(20)


Nucleoside Raw Materials

7-Deaza-2'-deoxy-7-iodoadenosine


In the precision race of Next-Generation Sequencing (NGS), we often focus on instruments and algorithms, but overlook the most fundamental cornerstone—the stability of the nucleotide molecules themselves.

When sequencing primers encounter complex high-GC regions or stubborn secondary structures, signal interruptions, soaring background noise, and sharply reduced read lengths become common. This is not just a loss of data, but also a silent consumption of time and cost.

I. When Sequencing Encounters "Stubborn Molecules"

Natural is not always optimal.

In the harsh sequencing environment of high temperatures and enzymatic reactions, the structural limitations of natural nucleotides (such as dATP) become apparent:

  • Insufficient Stability: Susceptible to environmental interference, leading to background noise.

  • Structural Rigidity: At complex template sites, it easily causes polymerase "stalling," resulting in shorter read lengths.

How to provide a more stable and "smoother" molecular foundation for the sequencing system? The answer lies in "structural remodeling" of the basic nucleotides.



II. One Tiny Modification, Three Major Breakthroughs

7-Deaza-7-iodo-2'-deoxyadenosine is precisely the molecular engineering masterpiece born for this purpose. It accomplishes two precise "micro-modifications" on the adenosine molecule:

  • Position 7 "Deaza": Replaces the N7 nitrogen atom in the adenine ring with a carbon atom, fundamentally enhancing the molecule's chemical stability and reducing undesirable interactions.

  • Position 7 "Iodination": Introduces an iodine atom, adding a highly reactive "chemical interface" to the molecule.

This dual modification brings about three synergistic scientific effects:

  • Conformational Stability: Improves Signal Quality

    The 7-deaza structure significantly reduces the base's tendency to protonate, allowing it to maintain conformational stability in complex biochemical reaction systems, thereby outputting sequencing data with higher signal-to-noise ratio and cleaner background.

  • Breaks Hairpin Bends: Improves Sequencing Uniformity

    Improves base stacking, helping the polymerase smoothly traverse high-GC regions, effectively increasing sequencing read length and coverage uniformity.

  • Highly Reactive Site: Enables Molecular Modification

    The iodine atom serves as a reaction site, enabling efficient conjugation with fluorophores, biotin, and other molecules.



III. Product Overview

166247-63-8 Structural Formula

Chinese Name: 7-Deaza-7-iodo-2'-deoxyadenosine

English Name: 7-Deaza-2'-deoxy-7-iodoadenosine

Abbreviation: Iodo dA

CAS Number: 166247-63-8

Molecular Formula: C11H13IN4O3

Molecular Weight: 376.15

Specification: 1 g / 25 g / 1 kg (custom packaging supported)

Form: Off-white powder

Product Number: G09010001

Iodo dA is a high-purity structurally modified nucleoside that can be directly applied to oligonucleotide synthesis or sequencing system construction. Its unique deaza and iodinated structure provides an ideal chemical unit for high-stability sequencing templates and functional nucleic acid design.



IV. Innovating from the Source, What Can It Do?

1. Next-Generation Sequencing System Optimization: Used to construct high-fidelity sequencing templates, reducing mismatched base signals;

2. Oligonucleotide Synthesis: Can be introduced as a modified monomer at specific sites;

3. Functional Nucleic Acid Research: The 7-position iodine group enables further substitution or conjugation reactions;

4. Probe and Drug Design: Provides highly reactive sites for nucleic acid probes or ApDC conjugates;



V. Why Choose Us?

✅ Molecular-Level Stability: Dual modification design lays a stable foundation for your system;

High Reactivity: The 7-position iodine atom provides an ideal reaction site for conjugation reactions;

✅ Strong System Compatibility: Compatible with mainstream DNA synthesis platforms and sequencing systems;

✅ Kilogram-Scale Stock Availability: Possesses kilogram-scale stable stock capacity, supports flexible custom packaging, helping you seamlessly connect R&D and production;




Extended Services

Iodo dA—Making sequencing more stable, design more free.

For high-quality nucleoside raw materials or custom packaging services, please call +86 13302967066!

Shenzhen GeneSeqTools Bioscience & Technology Co. Ltd. focuses on providing reliable domestic alternative solutions for gene sequencing, molecular diagnosis, and microbial detection.

For related products such as blood cell dyes and buffer salts, you can check the complete product line and technical specifications on the [MedSun Biotechnology Website].


In Vitro Molecular Diagnosis | Gene Sequencing | Microbial Detection
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Shenzhen GeneSeqTools Bioscience & Technology Co. Ltd.

Founded in 2015, it is a high-tech enterprise focusing on microbial testing gene sequencing and in vitro molecular diagnostics in the R&D, production, and sales of biochemical reagent raw materials. Committed to providing high-quality biochemical reagent raw materials and professional custom synthesis services, the product portfolio covers more than a dozen categories and nearly a thousand innovative products. Upholding the core philosophy of “customer first, integrity and professionalism”, we serve with dedication to meet customers’ diverse needs in research and testing.

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