Chinese Name: 4-Methylumbelliferyl-β-D-glucuronide

English Name: 4-Methylumbelliferyl-β-D-glucuronide

Abbreviation: MUG / 4MU-GlcA

CAS Number: 6160-80-1

Molecular Formula: C16H16O9

Molecular Weight: 352.30

Specifications: 25 mg / 100 mg / 500 mg (Custom packaging supported)

Form: Off-white crystalline powder

Product Number: G01070012

Stock: In stock

Fluorescence Signal: Blue fluorescence (excited by 365 nm UV light)

β-GUS recognition + fluorescence release: converting target bacterial characteristics into detectable signals.

MUG (4-Methylumbelliferyl-β-D-glucuronide) is a classic fluorescent substrate designed for β-D-glucuronidase. In enzyme substrate detection technology, its detection process mainly includes the following three steps:

1. Specific recognition

E. coli can produce β-D-glucuronidase. This enzyme has high substrate specificity and is an important enzymatic marker for identifying E. coli in water quality testing.

2. Enzymatic hydrolysis reaction

When β-GUS is present in the sample, the enzyme specifically hydrolyzes the β-D-glucuronide bond in MUG, releasing free 4-methylumbelliferone (4-MU).

Reaction principle:

MUG + β-GUS → 4-MU + D-glucuronic acid

3. Fluorescence signal generation

The released 4-MU has fluorescent properties, producing a blue fluorescence signal when excited by 365 nm UV light. Testing personnel can interpret results by observing fluorescence (qualitative) or using fluorescence detection instruments.

1. Environmental water quality testing

Suitable for the determination of E. coli in environmental samples such as surface water, groundwater, domestic sewage, and industrial wastewater.

Using the specific hydrolysis reaction of β-GUS on MUG, the fluorescence signal indicates the presence of the target bacteria, suitable for evaluating the sanitary status of environmental water bodies and related detection system development.

2. Detection of total coliforms in drinking water

Suitable for the detection and analysis of total coliforms in drinking water.

In the enzyme substrate detection system, MUG is often used together with total coliform detection substrates, generating differentiated detection signals through different enzymatic reactions, enabling the simultaneous detection and differentiation of total coliforms and target bacteria.

3. Detection of E. coli in drinking water

Suitable for the detection of E. coli in drinking water sources, tap water, and packaged drinking water samples.

As a classic fluorescent substrate in the enzyme substrate detection system, MUG converts β-GUS activity into a recognizable fluorescence signal, providing a reliable enzymatic detection method for drinking water microbiological hygiene indicators.

✅ High purity, reducing background interference

• High purity control: Purity ≥98%, strict control of impurity residues, ensuring low and controllable background signals.

• Controllable background: Effectively reduces non-specific fluorescence impact, significantly improving detection system stability.

• Batch consistency: Industrial-grade production standards ensure high reproducibility of test results across different product batches.

✅ Specific recognition, clear response

• Precise recognition: Uses β-D-glucuronide group as the recognition site, specifically hydrolyzed by β-GUS.

• Signal correlation: Fluorescence generation is directly related to enzyme activity.

• Clear detection: Clear reaction pathway, easy to establish standardized detection procedures.

✅ Fluorescence interpretation, convenient operation

• Classic fluorophore: Releases 4-MU upon enzymatic cleavage, producing a blue fluorescence signal.

• Intuitive reading: Typical detection conditions are excitation at 365 nm and emission at 445 nm.

• Equipment compatibility: Compatible with mainstream fluorescence microplate readers and detection platforms.

✅ Mature application, easy system development

• Wide application: As a classic fluorescent substrate in the β-GUS detection system, it has been widely used in E. coli detection systems in the water quality testing field.

✅ In-stock direct supply, ensuring supply rhythm

• Multiple specification inventory: Regularly stocked domestically to meet different scale needs.

• Controllable delivery time: Reduces the risk of cycle fluctuations caused by import dependence.

• Continuous supply: Supports stable supply from research validation to large-scale production.

In the enzyme substrate method, different detection indicators require matching different substrates. GeneSeqTools Bioscience & Technology provides a full range of substrate products related to water quality testing: