Chinese Name: 4-Methylumbelliferyl 2-acetamido-2-deoxy-β-D-galactopyranoside

English Name: 4-Methylumbelliferyl 2-acetamido-2-deoxy-β-D-galactopyranoside

Abbreviation: 4MU-GalNAc

CAS Number: 36476-29-6

Molecular Formula: C18H21NO8

Molecular Weight: 379.37

Specifications: 1 g / 5 g / 25 g (Custom packaging supported)

Form: Off-white crystalline powder

Product Number: G01070005

Stock: In stock

Signal: Blue fluorescence (4-MU)

4MU-GalNAc belongs to the 4-methylumbelliferyl class of fluorogenic glycosidase substrates, primarily used for detecting N-acetyl-β-D-galactosaminidase activity. Its detection principle is based on the "enzymatic hydrolysis – fluorescence release" mechanism: the substrate itself has weak fluorescence; after specific hydrolysis by the target enzyme, free 4-MU is released, generating a fluorescence signal under alkaline conditions, and the enzyme activity level can be evaluated by measuring changes in fluorescence intensity.

1. Substrate recognition and enzymatic hydrolysis

In the molecular structure of 4MU-GalNAc, 4-MU is linked to the N-acetyl-β-D-galactosamine moiety via a β-D-galactosaminide bond. When NAGase is present in the sample, the enzyme specifically recognizes and hydrolyzes the β-glycosidic bond, cleaving the N-acetyl-β-D-galactosamine group and releasing free 4-methylumbelliferone.

2. Fluorescence signal generation

Free 4-MU undergoes deprotonation under alkaline pH conditions, producing a blue fluorescence signal. The entire signal generation process is a one-step enzymatic reaction, requiring no coupled enzymes or chromogenic reagents, simplifying the workflow and facilitating fluorescence signal detection.

3. Signal quantification

Fluorescence intensity can be read at Ex 355 nm/Em 460 nm using a microplate reader or fluorescence spectrophotometer to monitor the production of 4-MU during the reaction. Under appropriate reaction conditions, changes in fluorescence signal can be used to evaluate NAGase activity and can be applied to the establishment and research of related detection systems.

✅ Targeting NAGase, supporting Candida albicans identification needs

• Target specificity: 4MU-GalNAc specifically recognizes NAGase, releasing detection signals through target enzyme hydrolysis.

• Species differentiation value: Detection of NAGase activity associated with Candida albicans can serve as an important reference indicator for its rapid identification, providing a basis for developing Candida identification methods.

• Reduced development validation difficulty: Clear enzyme-target relationships help companies establish rapid identification methods for Candida albicans, increasing the certainty of product development direction.

✅ 4-MU fluorescence signal output, enabling high-sensitivity quantitative detection

• Fluorescence signal release mechanism: The enzymatic cleavage releases 4-MU, producing enhanced fluorescence signal under detection conditions.

• Clear detection parameters: Detection wavelengths Ex 355 nm / Em 460 nm, compatible with common fluorescence detection platforms.

• Improved detection sensitivity: Fluorescence signals facilitate quantitative analysis, meeting the needs for trace enzyme activity detection and result evaluation.

✅ Compatible with high-throughput fluorescence detection, supporting rapid detection system construction

• 96-well plate compatible: The product can be used in 96-well plate fluorescence detection mode, suitable for batch sample analysis.

• Good platform adaptability: Fluorescence readout facilitates method development in combination with automated detection equipment.

• Easy to operate: Based on mature fluorescence detection systems, reducing operational complexity and improving detection consistency.

✅ Flexible supply, quality assurance

• Molecular biology grade purity: Strict batch-to-batch consistency control ensures reproducibility and reliability of experimental results.

• Stable delivery: Domestic in-stock supply, with performance comparable to imported products, shortening procurement cycles and supporting stable detection applications.

• Multiple specifications available: Available in 1 g / 5 g / 25 g, with custom packaging services also supported.